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HelpTest Code BTR Isoagglutinin Titer, Anti-B, Serum
Reporting Name
Isoagglutinin Titer, Anti-BUseful For
Evaluation of individuals with possible hypogammaglobulinemia
Method Name
Hemagglutination
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Serum RedShipping Instructions
Specimen must arrive within 10 days of collection.
Specimen Required
Supplies: Sarstedt 5 mL Aliquot Tube (T914)
Collection Container/Tube: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 2.5 mL
Pediatric Volume: 2 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum Red | Ambient (preferred) | 4 days | |
| Frozen | 10 days | ||
| Refrigerated | 10 days |
Reject Due To
| Gross hemolysis | OK |
Reference Values
Interpretation depends on clinical setting. No defined reference values.
Day(s) Performed
Monday through Friday, Sunday
CPT Code Information
86886
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| BTR | Isoagglutinin Titer, Anti-B | 34464-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| BTR | Isoagglutinin Titer, Anti-B | 34464-8 |
Secondary ID
113384Clinical Information
Isoagglutinins are antibodies produced by an individual that cause agglutination of red blood cells (RBC) in other individuals. People possess isoagglutinins directed toward the A or B antigen absent from their own RBC. For example, type A or O individuals will usually possess anti-B. The anti-B is formed in response to exposure to B-like antigenic structures found in ubiquitous non-red blood cell biologic entities (eg, bacteria).
Isoagglutinins present in the newborn are passively acquired from maternal circulation. Such passively acquired isoagglutinins will gradually disappear, and the infant will begin to produce isoagglutinins at 3 to 6 months of age.
Isoagglutinin production may vary in patients with certain pathologic conditions. Decreased levels of isoagglutinins may be seen in patients with acquired and congenital hypogammaglobulinemia and agammaglobulinemia.
Interpretation
The result is reported as antiglobulin phase, in general representing IgG antibody. The result is the reciprocal of the highest dilution up to 1:1024 at which macroscopic agglutination (1+) is observed. Dilutions above 1:1024 are reported as greater than 1024.
Cautions
Decreased isoagglutinin titers may be seen in normal elderly individuals and in children 12 months or younger.
This test will not be performed for individuals with blood type B or AB.
Clinical Reference
Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017
Method Description
Two-fold serial dilutions of patient's serum are tested with appropriate type A and B erythrocytes. Antiglobulin phase of reactivity is examined. The result is the reciprocal of the highest dilution at which macroscopic agglutination (1+) is observed up to greater than 1024. Parallel titration of control antiserum is used for standardization.(Fung MK, Eder AF, Spitalnik SL, Westhoff CM: Technical Manual. 19th ed. AABB; 2017)
Report Available
1 to 4 daysSpecimen Retention Time
14 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.Forms
If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.