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Test Code EXHD Hematologic Disorders, DNA Extract and Hold, Varies

Reporting Name

DNA Extract and Hold

Useful For

Reserving DNA on any specimen for which molecular analysis requiring DNA may be necessary at a future date, ensuring that adequate material for testing is available

Testing Algorithm

DNA will be extracted from the specimen and stored at -80° Celsius for one year from the date of the extraction process was performed. A report of "Performed" will be sent and a $50.00 processing fee will be assessed. To request molecular testing from the Molecular Hematopathology Laboratory's test menu, call 800-533-1710. Additional charges will be assessed for testing performed.

Method Name

Nucleic Acid Extraction

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Specimen Required


Specimen must arrive within 7 days of collection. Collect and package specimen as close to shipping time as possible.

 

Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.


Specimen Minimum Volume

Blood, Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred) 7 days PURPLE OR PINK TOP/EDTA
  Refrigerated  7 days PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Paraffin-embedded material
Fluids
Heparinized samples		 Reject
 

Reference Values

Not applicable

Day(s) Performed

Monday through Saturday

LOINC Code Information

Result ID Test Result Name Result LOINC Value
MP034 Specimen Type 31208-2
41995 DNA Extract and Hold Result No LOINC Needed
38305 DNA Extraction No LOINC Needed

Secondary ID

64779

Clinical Information

It is frequently useful to obtain DNA from clinical samples containing a hematopoietic neoplasm at the time of diagnosis so appropriate material is available for molecular analysis should subsequent testing be required. For example, when a diagnosis of acute myelogenous leukemia is made, there is a delay before karyotype information, which determines whether testing for molecular prognostic markers is necessary, is available. After this delay, the diagnostic sample is usually no longer available, or the DNA has degraded to such an extent that it is no longer adequate for testing. Thus, it is useful to obtain DNA on such specimens promptly at diagnosis and retain it until it is known whether additional testing is necessary.

Interpretation

A report of "Performed" will be sent and a $50 processing fee will be assessed. No interpretation will be given. Should the sample be used in future testing, interpretation would be incorporated with the final testing.

Cautions

DNA will be held at -80° Celsius for one year from the time of extraction. The DNA may only be used for suitable tests offered by the Molecular Hematopathology Laboratory at Mayo Clinic. It cannot be shipped to another institution for testing and cannot be released for research use.

 

Only liquid blood specimens will be processed. Paraffin-embedded material and fresh, solid tissue will not be accepted at this time.

Method Description

DNA is extracted using an automated system. The system includes a sample lysis step followed by binding of nucleic acid to a solid phase for separation from other cellular components and a final nucleic acid elution step.

Report Available

5 days

Specimen Retention Time

Blood/Bone marrow: 2 weeks; Extracted DNA:1 year

Test Classification

Not Applicable

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.