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HelpTest Code TEMZS Temazepam and Oxazepam, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Secondary ID
623017Useful For
Assessing compliance
Monitoring for appropriate therapeutic level
Assessing temazepam toxicity
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Temazepam and Oxazepam, SSpecimen Type
Serum RedSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Clinical Information
Temazepam is US Food and Drug Administration-approved to treat insomnia (trouble with sleeping). It is typically taken short-term (approximately 7-10 days). It is a benzodiazepine, which are a class of medicines that are central nervous system depressants and slow down the nervous system. It is extensively metabolized via conjugation and N-demethylation to oxazepam. Its elimination half-life ranges from 3.5 to 18.4 hours.
Reference Values
Therapeutic concentrations
Temazepam: 600-1100 ng/mL at 1 hour
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Temazepam: 10 ng/mL
Oxazepam: 10 ng/mL
Interpretation
Suggested therapeutic serum temazepam concentrations range between 600 and 1100 ng/mL at 1-hour post-dose.
Cautions
No significant cautionary statements
Clinical Reference
1. Langman LJ, Bechtel LK, Holstege CP. Clinical toxicology. In: Rifai N, Chiu RWK, Young I, Burnham CAD, Wittwer CT, eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elsevier; 2023:chap 43
2. Hiemke C, Bergemann N, Clement HW, et al. Consensus guidelines for therapeutic drug monitoring in neuropsychopharmacology: Update 2017. Pharmacopsychiatry. 2018;51(1-02):9-62
Method Description
The internal standard mixture containing chlordiazepoxide-d5, diazepam-d5, nordiazepam-d5, oxazepam-d5, and temazepam-d5 is added to serum samples, mixed, and centrifuged. The supernatant is diluted and injected on a liquid chromatography tandem mass spectrometer.(Unpublished Mayo method)
Day(s) Performed
Monday, Wednesday
Report Available
3 to 7 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80299
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TEMZS | Temazepam and Oxazepam, S | 11024-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 623017 | Temazepam | 10343-2 |
| 623018 | Oxazepam | 3886-9 |